Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.
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February 8, ; Accepted: Achromobacter, Actinobacter, Alcaligenes, Arthrobacter, Flavobacterium, Nocardia and Hydrocaarbonoclastic species are also hydrocarbon degraders Leachy et al.
This research is aimed at isolating hydrocarbonoclastic bacteria from petroleum refinery effluent and also assessing their bioremediating potentials in hydrocarbon cleanup programmes. Hydrocarbons are water insoluble and remain adsorbed to clay, humus particles and debris.
This finding is similar to the study carried out by Kafilzadeh et al. They persist in nature for long period of time and cause hazardous effects on flora and fauna of terrestrial and aquatic ecosystems.
The potential of oil-utilizing consortia bactsria with legume root nodules for cleaning oily soils. The treatment methods used for remediation include physical, chemical and biological processes. The growth curves in Fig.
Petroleum microbiology – Wikipedia
Table 1 and their respective degradative capabilities Figure 1. Support Center Support Center. Rehm HJ, Reiff I. The 3 species, Sphingomonas dokdonensisMarinobacter hydrocarbonoclasticusand Pseudomonas xanthomonas in Table 1 were not subjected to this analysis and, therefore, were absent in Table 2. In 20 years of research on the hydeocarbonoclastic in oil-polluted areas bacteriw the Gulf, we identified numerous bacterial species with the coupled potential for hydrocarbon mineralization and nitrogen fixation in many habitats including desert soil, seawater, hypersaline areas, rhizospheres, phyllospheres, and air dust, 124bacteriaa1617 Band I and Band II for every species were cut, amplified, and sequenced, and the sequences obtained were then compared with the nearest GenBank matches see Table 2.
This result corroborates with the report of Kaplan and Kitts Therefore, we assumed that, in bacteroa course of early evolution, microorganisms that developed the ability to utilize nitrogen-poor substrates, such as oil, simultaneously developed the potential for nitrogen fixation in order to synthesize their nitrogenous cell materials including proteins and nucleic acids.
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Atlas RM, Bartha R. Results and Discussion Growth on nitrogen-free medium and possession of nifH genes Eighty-two out of the hydrocarbonoclastic bacterial species tested were capable of growing on the NaNO 3 -free mineral medium with oil as the sole source of carbon and energy.
The probable reason why MCC was more effective than the pure isolates might be as a result of the synergistic effect of enzymatic reactions from various bacteria isolates on the refinery pollutant or the composite effect of enzymes of the five isolates acting more on metabolites than enzymes of individual isolates.
In the present study, we demonstrated that hydrocarbonoclastic bacteria isolated from all over Kuwait exhibited diazotrophic potential, as well as actual diazotrophic activity.
Indigenous species bloom after the hydrocarbon pollution. Mechanisms and occurrence of microbial oxidation of long-chain alkanes. Agriculture Bioinformatics Applications Biotech Products.
Hydrocarbonoclasyic of the bacterial isolates exhibited a lag phase in their growth performances. Crude oil consumption by individual bacterial species was measured quantitatively in batch cultures under the conditions specified above. Obligate oil-degrading Marine bacteria.
Crude oil and hydrocarbonoclashic degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait. This article has been cited by other articles in PMC.
Interest in this field is growing due to the increasing role of bioremediation in oil spill cleanup. Contact and attachment to the hydrocarbon molecule is thought to be pre-requisite step for oil degradation.
The bands of the 13 species tested revealed identical nifH gene sequences to those of heterotrophic bacteria in the GenBank database. Except for Pseudomonas aeruginosa whose optimal growth was attained from the 10 th to 16 th day, the other four bacterial isolates reached stationary and death phases after the 8 th to 14 th day of degradation of the crude oil Table 2. The results obtained from the identification were compared with Bergey Manual of Bacteriology Holt, A total of 36 distinct ITS haplotypes were detected on agarose matrix.
Neptunomonas naphthovorans degrade polyaromatic hydrocarbons PAH especially naphthalene. They have been exploited for their biodegradation potential and have been used successfully for cleanup of oil contaminated soils and aquatic systems. This page was last edited on 26 Juneat Indigenous as well as in vitro formulated consortia of HCB are being used hydrocarbonocllastic only for oil spill mitigation hydrocarbonockastic also for biopolymer production and in biocatalysis.
Journal List Microbes Environ v.
The tested species also grew well and consumed crude oil effectively in NaNO 3 -containing medium with and without nitrogen gas in the top space. To hydrocarbknoclastic such risk, bioremediation constitutes an environmentally friendly alternative technology that has been established and applied. The vegetation-poor desert soil and Arabian Gulf water body are also poor in nitrogenous compounds.
Microbiological nitrogen fixation is an activity that requires large amounts of energy. Hydrocarbons are recalcitrant because of their oily nature and poor water solubility. They are heterotrophic and can grow efficiently in presence of aliphatic and aromatic hydrocarbons. Based on their diazotrophy, such bacterial species have the ecological advantage of survival in environments extremely poor in or free of nitrogenous compounds. Culture-independent approach DGGE showed high diversity in the microbial community in all the studied samples with a clear correlation with the hydrocarbon pollution rate.
The novelty of this study is not only related to its methodology, but also to its major findings. They always grow in environment of high carbon and low nitrogen content which is ideally created by hydrocarbon pollution.
Crude oils are composed of an array of chemical compounds, minor constituents, and trace metals.